Protein Characterization

Single Protein Analysis
JadeBio can provide in depth characterization of purified proteins from relatively simple mixtures to provide information on sites of modification or impurities in preparations.


Immunoprecipitation Characterization
A proteomics read out from an immunoprecipitation study can provide information on co-immunoprecipitating proteins that may be part of complexes or for characterizing the target protein(s) of an antibody.

Antibody Sequencing
Partially or completely unknown sequences of antibodies can be determined and codon optimized DNA sequence can be returned for a target species expression system. Also, multiple antibodies arising from the same immunization can be quickly screened for sibling sequences through partial proteomic sequencing to reduce wet lab characterization efforts and speed cloning.

Antibody Drug Conjugates (ADCs)
We can identify the sites of conjugation and quantify the stochiometry of the conjugations.


Analysis
Technology Platform Turnaround Time
Peptide mapping 1D nano LC-MS/MS with multi-enzyme digestion up to 4 samples/week
Protein identification 1D nano LC-MS/MS with one enzyme digestion 5 samples/week
Intact protein analysis 1D LC MS 500 samples/week
CoIP or pull-down - label free 1 day, 3-Phase 2-Dimesional nano LC MS/MS, relative quantitation with spectral counting 5 samples/week
CoIP or pull-down -iTRAQ 1 day, 3-Phase 2-Dimesional nano LC MS/MS, relative quantitation with spectral counting 2 sets/week
Germline screening 1D nano LC-MS/MS 24 samples/week
Sequence variance identification 1D nano LC-MS/MS with multi-enzyme digestion and multi-step data analysis 1 sample/3 weeks
Antibody identification and sequence confirmation 1D nano LC-MS/MS with multi-enzyme digestion up to 4 samples/week
Unknown mAb sequencing 1D nano LC-MS/MS with multi-enzyme digestion and multi-step data analysis 1 sample/3 weeks
ADC intact mass 1D LC MS 500 samples/week
ADC conjugation sites/quantitation 1D UPLC-SRM with or without internal standards 500 samples/week
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